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We report on sensitivity, reproducibility, concordance, DNA mixtures and carryover for EXT sample cartridges pre-loaded with GlobalFiler ® Express and AmpFLSTR ® NGM SElect™ Express, (Thermo Fisher Scientific, Waltham, MA) STR chemistries. Once the sample is loaded in the RapidHIT EXT sample cartridge, a DNA profile is produced after amplification, detection and automated data analysis.
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RapidHIT EXT sample cartridge allows a user to generate a forensic DNA profile from less than 250 pg of extracted and quantified DNA in less than 90 min with less than one-minute hands-on time. A new generation of the RapidHIT sample cartridge for RapidHIT ID has been designed and tested which allows the loading of extracted and quantified DNA. RapidHIT ® 200 and RapidHIT ID are rapid DNA systems developed by IntegenX (Pleasanton, CA) and validated for the use of buccal swabs.
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Features of the Applied Biosystems RapidHIT ID System include: Fast 90-minute system run time.
Integenx rapidlink software#
This system is for use with Applied Biosystems RapidLINK Software v1.0 and its v1.0 applications. Rapid DNA Analysis is a hands-free “swab in – profile out” process which consists of automated DNA extraction, amplification, separation, detection, and allele calling without human intervention. IntegenX Inc., leader mondiale nellidentificazione rapida del DNA umano, plaude allapprovazione di S.139 e HR.510, ossia la Legge sullidentificazione rapida del DNA del 2017. The RapidHIT ID System delivers the reliable, reproducible, high-quality data you have come to expect from the Applied Biosystems brand.
Integenx rapidlink manual#
However, PCR amplification and electrophoretic analysis remain largely manual. RapidLINK software (IntegenX) for processing, including manual profile review (Figure 3). Routine DNA extraction and quantitation is readily available. Simplification and integration of the routine steps would dramatically improve turn-around time and reduce the risk of operator contamination. Crime scene DNA sample processing involves both complex and routine processing steps. The turn-around time of urgent crime scene DNA samples is often far longer than desired by law enforcement.